EMP DNA Extraction Protocol (current)

View the protocol on protocols.io: doi.org/10.17504/protocols.io.pdmdi46

Please cite Marotz et al. (2017) if you use the EMP DNA Extraction Protocol in your research.

EMP DNA Extraction Protocol (previous)

View the protocol on protocols.io: https://doi.org/10.17504/protocols.io.nutdewn

KatharoSeq: DNA extraction from low-biomass samples

KatharoSeq (Minich et al., 2018) is designed for low-biomass samples, incorporating positive and negative controls and bioinformatic methods to identify differences in microbial communities with as few as 50 to 500 cells.

Please cite Minich et al. (2018) if you use KatharoSeq in your research.

Next steps

References

  • Marotz, C., Amir, A., Humphrey, G., Gaffney, J., Gogul, G., & Knight, R. (2017). DNA extraction for streamlined metagenomics of diverse environmental samples. BioTechniques, 62(6), 290–293. http://doi.org/10.2144/000114559
  • Minich, J. J., Zhu, Q., Janssen, S., Hendrickson, R., Amir, A., Vetter, R., et al. (2018). KatharoSeq enables high-throughput microbiome analysis from low-biomass samples. mSystems, 3(3), e00218–17. http://doi.org/10.1128/mSystems.00218-17

Change log

  • August 2018 – Added links to current and old protocols on protocols.io.
  • December 2018 – Added reference to low-biomass sample input protocol (Minich et al., 2018).